Spread Plate TechniqueAccording to Sagar Anyal (2016), Spread plate technique is the method of isolation and enumeration of microorganisms in a fixed culture, and distributing it evenly. The technique separates microbes contained within a small sample volume which is spread over the surface of an agar plate, using a sterile L-shaped glass rod to ensure even distribution of the colonies in order for them to form evenly on the agar
